Our lab has always used a laser based (Sutter P-2000) needle puller to pull our needles for microinjection. Our machine broke and the only other needle pullers we have access to are filament based. We have access to either a Sutter P-87 or P-97 instrument, but so far I have been unsuccessful in figuring out what parameters to use. Has anybody used one of these models to pull their needles and if so could you let me know what settings you use.