I am looking for suggestions for a non-radioactive northern blot kit that has worked well. Any comments about the sensitivity of current-generation kits compared to 32P would be welcome, too. I don’t currently have a radioactivity license and want to avoid going through the hassle if I can do so without sacrificing sensitivity.
I have also heard that you can’t strip and reprobe non-radioactive northerns. Are you able to probe once and then reprobe (without stripping) with a loading control like rpa-1 that should be smaller than my band of interest?
Finally, I don’t have an X-ray film developer. I can use either our Bio-Rad ChemiDoc to scan the blot or expose it to a phosphor screen and read it with a Bio-Rad Storm imager. Anyone have experience with either of these methods?
Thanks,
Tim