The November Papers with Technical Advances are here: https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Nov_2025. Don’t forget to flag when you Fast-Track Your Paper if you want your paper with a new technique, reagent, or resource featured next month.
Long-read sequencing-based analyses of the adult Drosophila brain transcriptome in physiological and pathological settings. (BMC Genomics)
Oxford nanopore direct RNA long read sequencing of six biological replicates.
https://pubmed.ncbi.nlm.nih.gov/41087881
Improved detection of lipidated Atg8a by immunoblotting in Drosophila melanogaster cells and tissues enables precise investigation of Atg8a flux and its termination. (Autophagy)
Refined approach to Atg8 immunoblotting that employs N-ethylmaleimide (NEM), thereby preserving Atg8a lipidation during sample preparation both in vitro and in vivo. We determined the optimal concentration of the autophagic inhibitors bafilomycin A1 (BafA1) and chloroquine (CQ).
https://pubmed.ncbi.nlm.nih.gov/40426043
Ribozyme-Mediated Knockdown of lncRNA Gene Expression in Drosophila. (Bio Protoc.)
Targeted insertion of the N79 ribozyme into Drosophila genes by CRISPR-mediated genome editing. The ribozyme causes rapid and complete destruction of mRNAs or lncRNAs in which it is co-transcribed
https://pubmed.ncbi.nlm.nih.gov/41141636
Multilayer regulation underlies the functional precision and evolutionary potential of the olfactory system. (Nat. Commun.)
We generate a single-cell transcriptomic atlas of all developing antennal sensory lineages of D. melanogaster, including neural populations that normally undergo programmed cell death.
https://pubmed.ncbi.nlm.nih.gov/41152261
Reevaluation of whether histones are asymmetrically segregated during asymmetric divisions of stem cells in Drosophila. (Proc. Natl. Acad. Sci. U.S.A.)
Photoswitching the Dendra2 using in vivo intravital imaging strategy FlyVAB.
https://pubmed.ncbi.nlm.nih.gov/41166424
Intracellular lactate dynamics in Drosophila neurons. (iScience)
This paper employs machine learning to predict metabolic responses of Drosophila neurons to metabolic inhibitors and substrates, as well as custom software to correct real-time the effects of pH on fluorescent metabolic biosensor readouts.
https://pubmed.ncbi.nlm.nih.gov/41169509
Protocol for evaluating protein intake-induced feeding inhibition in Drosophila using a pre-feeding approach. (STAR Protoc)
This optogenetic pre-feeding protocol is designed to study the neural mechanisms underlying protein intake-induced feeding inhibition (PIFI) in Drosophila. The amount of food intake and excretion of flies is assessed under natural feeding conditions without anesthesia.
https://pubmed.ncbi.nlm.nih.gov/40906553
Resolving early embryonic metabolism in Drosophila through single-embryo metabolomics and transcriptomics. (Nat Metab)
Single-embryo transcriptomics and metabolomics
https://pubmed.ncbi.nlm.nih.gov/40804333
Layers of immunity: Deconstructing the Drosophila effector response. (eLife)
A new set of transgenic fly lines has been deposited in the VDRC tied to this study for the investigation of immunity: https://shop.vbc.ac.at/vdrc_store/drosdel-info In addition, while no new alleles were generated, a number of novel combinations were made and deposited.