G3 | January 2024 | Vol. 14, Iss. 1
Humanization reveals pervasive incompatibility of yeast and human kinetochore components
Guðjón Ólafsson, Max A.B. Haase, Jef D. Boeke
Ólafsson et al. show that CENP-A or yeast–human chimeras cannot replace Cse4p, even in the context of human histones, suggesting that human CENP-A can be incorporated into budding yeast chromatin but does not support native kinetochore function. The authors highlight differences between the centromeres and kinetochores of humans and budding yeast, as well as underappreciated similarities in epigenetic regulation of centromeric function.
