Hi all,
May i know if anyone had came across with how to over-express the gene downstream of DAF-16?
what i have in hand is that, we do learn that RNAi of daf-2 would in turn lead to activation of DAF-16/FOXO transcription factor, which in turn would lead to up-regulation/expression of the daf-16 downstream genes.
but we wonder if there is any other method that would do the same trick?
i have receive suggestion, such as to use some kinase (AKT-1, PDK-1) inhibitor in the upstream of daf-16, but i really dont know where should i start to look from.
any input will be much appreciated. Thanks.
cheers,
tyrael