How exactly do you select for pha-1 rescue after you inject pha-1(e2123ts) with pBX?
Do you just put the injected adults into 25 degrees? Or how long do you let them recover at 16 degrees before shifting to 25?
You can put them at 25°C, although the selection works well in our 20°C incubator, so I do that. The non-rescued animals aren’t as dead, but they are well enough behind that the rescued guys are the only animals that reach adulthood.