phospho-p38 antibody

Hi all,

I’m new here. I hope you guys can help me. I’ve been trying to detect the phosphorylated p38 MAPK (Promega) from native protein samples, but I just can’t make it to work. The anti-PMK-1 and the anti-beta tubulin seems to work fine. Any suggestions?

FYI I dilute the antibody (1:1000) in 5% skim milk+PBS solution, incubate them overnight at 4oC, and used anti-rabbit secondary antibody.


In my lab, Phospho-p38 MAPK (Cell Signaling #9211) is working well.
We dilute the antibody 1:1000 in 5% skim milk + TBS-T.