Hi all,
I’m new here. I hope you guys can help me. I’ve been trying to detect the phosphorylated p38 MAPK (Promega) from native protein samples, but I just can’t make it to work. The anti-PMK-1 and the anti-beta tubulin seems to work fine. Any suggestions?
FYI I dilute the antibody (1:1000) in 5% skim milk+PBS solution, incubate them overnight at 4oC, and used anti-rabbit secondary antibody.
Thanks!!