Pofm-1::gfp - specificity of coelomocyte expression..?

Model Organism: Worms Methods & Reagents
1

Want to confirm my goiP::mCherry & known duct cell marker lin-48P::gfp transgenes overlap in duct cell. Don’t want to use rol-6 as a marker
so was looking at Pofm-1::gfp. How specific (and bright) is expression in coelomocytes (obviously want duct cell gfp to be due only to lin48P)?

2

Hi there

Unc-122 works beautifully as coelomocyte marker. Specific and bright.

https://www.addgene.org/19325/

Hope it helps