Hi. Does anyone have a primer sequence for a really reliable positive PCR control for C. elegans genomic DNA? I need something that works every time.
Pretty much every primer pair I’ve ever found using Primer3 for a 4-600 bp product from elegans genomic DNA has worked (every time, from crude lysate, without having to do nested PCR).
If you want something that’s been used by many people lots of times, there are primer pairs for snip-SNP genotyping - see Davis et al, for example.