I always observe precipitating crystals in my liquid culture S-medium after addition of op50 pellet.
During synchronization there is no precipitate, no matter how long I leave the worms, but after some time with bacteria there is precipitation.
Might be due to some LB medium constituents or due to lysed bacteria, which I usually resuspend in S-medium or M9???
Thanks for any type of advice,
Marlon