pRF4 sequence?

Model Organism: Worms Methods & Reagents
1

how to get the sequence of pRF4??
wish someone can help me. :-[

2

  1. go to http://www.ncbi.nlm.nih.gov/

  2. Chose from the ‘Search’ - menu “Protein” or “Nucleotide” (depending on which sequence you need).

  3. Enter your search term (in your case pRF4)

  4. click on “GO”

  5. Select the sequence/organism, you need (BUT try to avoid choosing “chromosome xyz, complete sequence”, as it takes ages to download).

  6. Scroll down to the bottom of the page.

3

Thanks for your reply.

pRF4 is a worm expression vector , a kind of co injection marker, but not a gene or protein.
Actually,I want to get the sequence of pRF4 vector.

4

Hi,

I don’t have the sequence for you, but as far as I know pRF4 is a ±4kb EcoRI fragment of rol-6 into pUC19 (ref: Mello, Kramer, Stinchcomb, Ambros, EMBO journal(1991) Efficient gene transfer in C. elegans). You could try to put it together in silico yourself.

Best,
Josien

5

Thank you very much!