Good day everyone.
I still cant get the neuron and muscle cell in large quantities since I need it for treatment later.
I already use cover slip coated with peanut lectin. But still the embryo not differ into my interest cell.
Is it possible if I try culture the embryo cell on the pre-treated poly-L-lysine@peanut lectin plastic culture dish?
Maybe I can increase the space for the cell to adhere and differentiate by using culture dish that already coated rather than only using cover slip?
Or is it A Must to use glass culture vessel?
Hope your kindly sharing here. Thank you in advance.
OK, I can appreciate that having to ask questions in English when it’s not your native language can be a problem…I live in Germany and knock the hell out of the German language everyday…however, I think you could make your question simpler and clearer…and then probably more people would reply.
HOWEVER, your post jogged something in my memory and I looked through my earlier posts and found this;
http://forums.wormbase.org/index.php?topic=1523.msg3257#msg3257
Did you follow / try Steve von Stetina’s excellent suggestion at all? ???
steve
Sorry for late reply. Thank you for reminding me about that post.
Recent paper published did help me on embryonic culture http://www.wormbook.org/chapters/www_cellculture/cellculture.pdf 