I am working on C.elegans chemotaxis. i seem to have run into a problem. I am trying to reproduce this paper
http://www.ncbi.nlm.nih.gov/pubmed/21445035
Unfortunately i am not able to replicate it. Just for standardization purpose. I am just working with naive and 1 h starved worms.
Although my starved worms have shown a slight increase (as reported), i am getting a chemotaxis index of 0.4-0.5 instead of the reported 0.28-0.30.
WHat might be the problem?