Promoter for marginal cells

Model Organism: Worms Scientific Discussion
1

Dear all,

Does anyone of you know a promoter that is specific for the pharyngeal marginal cells? I aim at expressing a rescue construct in these cells as it seems that my gene of interest is strongly expressed there. The only promoter i was able to find is ttx-1; however, this doesn’t work well for me.
I am now looking for alternatives but cannot seem to find any. I guess a promoter that is expressed in a few other cells would do the job, too.

Any help would be greatly appreciated!

Best,
sue

2

Hi Sue,

I personally don’t know of any, but if you poke around in WormBase you can see what genes have been reported to be expressed in the marginal cells (Associations tab).

http://www.wormbase.org/species/all/anatomy_term/WBbt:0003673#01--10

edit: also, if you check out the pharynx chapter on WormAtlas, Figure 6/7 may help :slight_smile:

3

Hi Snug,

Thanks for your reply!
I didn’t know of this specific function in Wormbase, so thanks for pointing it out. :slight_smile:
Luckily, it seems as if there were some possibilities, but i will look more carefully into it.

Thanks again,
sue