question about the history of gene mapping/cloning in C. elegans

[mikechao]

hello, this may be a somewhat naive question: in the old days when the genetic/physical map was still not well developed, how were the first mutants mapped to physical cosmid clones? In other words, given a nice genetic map full of Uncs and Dpys and so forth, how do you then correlate that to a specific clone in a contig library?

thanks,
Mike

[raymond]

one had to make their own contig in the olden days by chromosomal crawl, walk and jump. example: http://wormbase.org/db/misc/paper?name=WBPaper00001107;class=Paper. for those who can’t get the full article easily, here’s a WBG article that gives the gist http://wormbase.org/db/misc/paper?name=WBPaper00016228;class=Paper.

raymond

@mikechao post:1

hello, this may be a somewhat naive question: in the old days when the genetic/physical map was still not well developed, how were the first mutants mapped to physical cosmid clones? In other words, given a nice genetic map full of Uncs and Dpys and so forth, how do you then correlate that to a specific clone in a contig library?

thanks,
Mike