Hello
New to c elegans and research and very confused.
Would like to expose c elegans to glucose of varying concentrations and observe effects of it (fairly basic experiment…)
How do I expose the c elegans to glucose? Do I add it to the plates before the OP 50? Any protocols would help. I am planning on exposing it for 3 days, is that sufficient?
Should I expose it to glucose at the adult stage and also larval stage? I am already planning on doing ( young) adult stage.