Dear all,
I’m new to this web site. I’m a structural biologist. I am interested in some proteins with overlap between species. I may be moving to Brazil in the near future, and am trying to acquire several things to get started.
My main problem is cheep or free cDNA for c.elegans, and then I am also looking for protocols in large scale worm growth (normal conditions, normal or over expressed protein clones, etc) with the aim of protein purification. In a similar manner, I am interested in nuclear extraction protocols (or leftovers if anyone wants to give them up), as human nuclear extracts are pricy and hard to get enough for structural work if done in the lab. Basically, I would like to have 200 ml at 2 mg/ml protein conc. nuclear extract in the end, from worm culture. I need however, to find out feasibility of such things, as I only know worm protocols from several courses, but have not used them much directly (other than a protein or two in e.coli). Any suggestions or comments (or supplies) are appreciated, and I would gladly give the Brazilian lab address if anyone needs. I most likely will post other questions and searches for material here in the future.
mit freundlichen Grüssen,
Stephan Watkins