Hey everyone,
Science Fair is less than a week away and I’ve just finished up my project. I appreciate all the help you’ve given. I have a few more questions though, and I had trouble finding the answers online:
- Do any pathogenic bacteria (to C. elegans) attack the dopamine neurons of the nematodes?
- Can fluorescence vary by size? What about from worm to worm?
- Should the fluorescence appear in the gut area, and if so, why?
- In one study I can across, the authors said they used BZ555 egls[Pdat-1::GFP]. What does “egls[Pdat-1::GFP]” mean?
Thanks!
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I’m not certain - perhaps someone else on the forum can weigh in.
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I’m assuming you are talking about a transgene. Fluorescence does vary by size and from worm to worm. Integrated arrays are more consistent in expression across a population of worms than are extrachromosomal arrays.
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The gut does autofluoresce.
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egIs is an integrated transgene array expressing GFP under the dat-1 promoter:
http://www.wormbase.org/db/gene/transgene?name=egIs1;class=Transgene
The nomenclature guide is here:
http://www.wormbase.org/wiki/index.php/UserGuide:Nomenclature#Transgenes