RNA seq

Model Organism: Worms Methods & Reagents
1

Hi there,

I am in the process of getting a protocol done for RNA-seq using the illumina platform. This is my first shot at this experiment and like most times I am stuck with a bunch of queries.

  1. Is there a kit with which I can process both smallRNA and total mRNA for RNA seq (I guess not, but worth asking as suppose)
  2. Are the adapters for the illumina kits identical/interchangeable (truseq and mRNA seq8 kits)
  3. The best multiplexing option/kit or could I use the index’s from the truseq kit on samples processed by the mRNA seq8 kit.

Help will be much appreciated.
Many Thanks :slight_smile: