In the Fitch lab we are interested in the RNAscope technology that amplifies mRNA signal allowing for visualizing the subcellular localization of RNA molecules. Although we do not necessarily need to
quantify the transcript abundance, as this gene is highly expressed, we would like to have a unambiguous answer about where the RNA is within the cell.
Has anyone in the worm community worked with ACD and their RNAscope technology and are willing to discuss methods with us?
Our RNA of interest is ~600nt long in its spliced form, which is possibly too short for other methods (i.e. smFISH) to be effective or straightforward.
Any tips or help are greatly appreciated!
Antonio