This message was sent by Khillan Jaspal NIH/NIAID [C] email@example.com
We, at the National Institute of Allergy and Infectious Disease (NIAID), have an opening for a Molecular Biology expert for creating CRISPR/cas9 genetically modified animal models. Please forward this information to your colleagues or someone who might be interested. I will greatly appreciate your help.
Jaspal S. Khillan Ph.D.
Mouse Genetics and Gene Modification Section
Rm. 1E 12C3 Bldg. TB3
12735 Twinbrook Parkway
Rockville MD 20852
Research Support Specialist IV – MGGM
Requirements: Candidate must have a bachelor’s degree with 3 to 5 years of lab experience in molecular biology, genetics, biochemistry or related fields, a MS/PhD. degree preferred. Must have experience in applying genetics knowledge to the laboratory animal field especially relate to genetically engineered mice (GEM) including embryo manipulations, gene targeting in embryonic stem (ES) cells and development of gene constructs for production of GEM. Candidate must have experience in in vitro fertilization (IVF), as well as rodent technical procedures and colony management. Proficiency in MS Access/Outlook/Word/Excel is required.
Overview: The person will be responsible for creating genetically modified gene knockout /gene knock-in mice via CRISPR/cas9, preparation of plasmid DNA and RNA, maintaining animal colonies, embryo and sperm cryopreservation, in vitro fertilization (IVF) and rederivation of lines.
Description of Duties:
• Perform genome modification of mice via CRISPR/cas9 microinjections in embryos to create gene Knock out and gene knock in animals, production of transgenic mice via pronuclear injection.
• Perform injections of gene targeted embryonic stem (ES) cells into blastocysts to create chimeric mice.
• Assist with the cryopreservation of mouse germplasm, including freezing and storage of sperm.
• Rederive of lines from frozen embryos and sperm via in vitro fertilization, maintaining of animal colonies to augment the existing cryopreservation and rederivation program.
• Provide rodent genotyping assistance by collecting samples from gene modified animals.
• Prepare tissue, and germplasm samples for transport and storage utilizing liquid nitrogen.
• Maintain liquid nitrogen freezers for stored samples, checking liquid nitrogen levels and refill as necessary.
• Maintain integrity of the embryo rederivation efforts, including detailed accurate record keeping and routine breeder observations.
• Report any animal health, care, and/or well-being concerns to the appropriate facility management staff.
• Ability to work flexible hours and with other team members to ensure research needs and experiments are carried out to comply with appropriate timelines.
• Perform other duties as assigned.