I know usual advice is to add sheared worm gDNA in injection mixes to help generate ‘complex’ arrays of DNA but just wondering whether anyone has any experience of using
other sources of gDNA instead? I’m going to try sheared salmon sperm DNA as we have some in lab.
you can also try using DNA ladder, convenient and easy to bring your final concentration of your DNA mix to at least 100ng/uL
you can also use OP50 gDNA at 100ng/uL. We use this successfully our the lab