Hello all friends,
Glad to have a communication with every one!Recently,I’looking for a transgenic strain—aak-2::gfp overexpress,but always found none.I AM a newer in this field.
Is there any expert who could help me find it?OR who has it?I can pay for it. 
Maybe some information for its cDNA clone is also helpful for me.
Plenty thanks! looking forward to reply soon!
Hi,
you mean something like this strain? It’s available from the CGC.
http://www.wormbase.org/species/c_elegans/strain/BC10615#02--10
Steve
Hmm, actually I think she’s looking for a translational fusion and not a transcriptional fusion. You should check out this reference.
Thank you very much ,Steve.I have searched this strain before by a reference.BUT I do not understand whether it can express a AAK-2::GFP protein.Frankly
speaking, I need a protein AAK-2.Due to my poor knowledge and technique about C.elegans,it’s hard for me to overexpress a AAK-2 protein through C.elegans directly .Besides,I found antibodies to C.elegans is not easily available.
As a result,I think out this way —seek a AAK-2::GFP overexpressing strain,and immunoprecipitate it by an antibody to GFP,as the reference Snug mentioned,which I have read before.
I really looking forward to a help. 
So if you read the reference then what’s the problem? The authors describe the generation of an integrated array, fyIs2, which is what you’re looking for.
fyIs2[AAK-2-GFP 100 ng/mL] was generated by integration of the extrachromosomal array by irradiation with ultraviolet light (254 nm) and out-crossed four times to wild type. fyIs2 fully rescues the Daf-d phenotype of daf-2(e1368); aak-2(ok524) at 25°C.
source: the reference I linked
BEFORE it was a little like this clip from black adder…
http://www.youtube.com/watch?v=sl3IW-pPMyU
Now clarity cuts its illuminating path…you want a fusion protein.
As Snug says…it does exactly what it says in the reference.
Good luck…
Steve