The FlyBase Papers with Technical Advances page has updated with papers submitted in September.
If you’d like to be featured in an upcoming update, please flag your paper with a new resource, technique, or reagent when you Fast-Track Your Paper.
A bidirectional brain-fat body axis for pathogen avoidance. (Neuron)
In vivo fat body functional imaging method
https://pubmed.ncbi.nlm.nih.gov/41997146
Zasp52’s differentially expressed intrinsically disordered region confers thin filament stability at the Z-disc. (eLife)
Fluorescence recovery after photobleaching (FRAP) in indirect flight muscle
https://pubmed.ncbi.nlm.nih.gov/42657781
Regulatory logic of neuronal differentiation in the Drosophila visual system. (Proc. Natl. Acad. Sci. U.S.A.)
Comprehensive single-cell multiome (RNA+ATAC-seq) atlas of the developing visual system at 4 stages.
https://pubmed.ncbi.nlm.nih.gov/42616773
Beat- and Side-family cell-surface molecules are expressed combinatorially in the partner neurons of the olfactory circuit in Drosophila. (PLoS Biol.)
This study reported a collection of MiMIC-based T2A-GAL4 reagents for almost all beat/side genes, which enables the systematic characterization of expression and function of these genes in diverse contexts of neural development.
https://pubmed.ncbi.nlm.nih.gov/42659622
A centroid-based video-tracking framework for integrated analysis of sleep, locomotor activity, and spatial behavior in Drosophila melanogaster. (J. Neurosci. Methods)
A validated, no-code ANY-maze workflow and ready-to-use configuration for integrated video-based analysis of sleep, locomotor activity, temporal movement patterns, and spatial occupancy in Drosophila.
https://pubmed.ncbi.nlm.nih.gov/42508502
EC-isHCR: A rapid method for in situ hybridization chain reaction in diverse animal samples. (Methods)
EC-isHCR enables rapid RNA detection but has been validated only in a limited range of samples. Here, we show that EC-isHCR robustly detects RNA across diverse Drosophila samples.
https://pubmed.ncbi.nlm.nih.gov/42372957
Metabolic and developmental rate divergence between serially homologous cells underlies an evolutionary innovation in Drosophila. (PLoS Biol.)
Time-series single-cell RNA-seq atlas of the development of the sensory organs (and sex comb) on the first tarsal segment.
https://pubmed.ncbi.nlm.nih.gov/42636207
Membrane lipid composition and rapid endocytic retrieval modulate Wingless secretion. (EMBO J.)
- wg-GalDBD-nMag, wg-Gal80 & hh-Gal80 for specific Gal4 inhibition 2) endogenous GFP-tagged clathrin + optogenetic tools for light-controlled acute reversible knock-sideways 3) endogenous SNAP-Wg for SNAP-tag labelling 4) endogenous FRT-schlank-HA-FRT for schlank labelling & cKO
https://pubmed.ncbi.nlm.nih.gov/42547564
Evidence for dual pathways of Tc1/mariner domestication in Drosophila. (BMC Biol.)
The paper provides a stringent multi-criteria framework for identifying bona fide TE domestication events across 43 drosophilid genomes, distinguishing functional domesticated genes from decaying pseudogenes by integrating synteny, selection, structure, and expression analyses.
https://pubmed.ncbi.nlm.nih.gov/42298553
Assessment of adult structural plasticity in Drosophila neurons. (Open Biol.)
We identify key sources of variability in adult neuronal remodelling and provide both an optimized protocol and MorphoScope, an open-source Python tool for unbiased quantification of arbour complexity, applicable to different models.
https://pubmed.ncbi.nlm.nih.gov/42710896
Arginine Kinase 1 supports energy homeostasis in Drosophila flight muscle development. (PLoS Genet.)
single cell RNA-seq dataset in wing discs of third instar larvae using 10x Genomics platform.
https://pubmed.ncbi.nlm.nih.gov/42752582
Dimethyl sulfoxide perturbs sleep-related behavioral organization in multiple species and confounds interpretation of sleep phenotypes. (Front Toxicol)
Specify the appropriate concentration of DMSO for drug-administration experiments, such as those in sleep research.