September updates to the FlyBase Papers with Technical Advances page

The September Papers with Technical Advances are here: https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Sep_2025. Don’t forget to flag when you Fast-Track Your Paper if you want your paper with a new technique, reagent, or resource featured next month.

A Drosophila single-cell 3D spatiotemporal multi-omics atlas unveils panoramic key regulators of cell-type differentiation. (Cell)

This paper generated a comprehensive single-cell multi-omics database including scRNA-seq and scATAC-seq data spanning embryogenesis, as well as spatial transcriptomic data from embryos to pupae. This database provides a useful resource for Drosophila research.

https://pubmed.ncbi.nlm.nih.gov/40578340

Establishment of Drosophila intestinal cell lines as tools for multiomic screening and deciphering intestinal biology. (Sci. Rep.)

Intestinal derived cell lines from D. melanogaster

https://pubmed.ncbi.nlm.nih.gov/40897748

Standard ingredient of Drosophila medium reduces transmission and virulence of the gut pathogen Pseudomonas entomophila. (Microbiol. Spectr.)

We evidence how Nipagin (methylparaben), a very common additive in fly food, critically affects the response to bacterial infection

https://pubmed.ncbi.nlm.nih.gov/40793765

Drosophila video-assisted activity monitor (DrosoVAM): a versatile method for behaviour monitoring. (R. Soc. Open Sci.)

A new, video-based fly monitoring system is developed to monitor fly activity. The system is relatively inexpensive, simple to implement, and can be modified for use in a variety of different assays including the monitoring of circadian activity and conditional preferences.

https://pubmed.ncbi.nlm.nih.gov/40919370

Hosts and Commensal Bacteria Synergistically Antagonize Opportunistic Pathogens at the Single-Cell Resolution. (Adv Sci (Weinh))

Bacterial Single-cell RNA-seq

https://pubmed.ncbi.nlm.nih.gov/40407187

Monoamine-induced diacylglycerol signaling rapidly accumulates Unc13 in nanoclusters for fast presynaptic potentiation. (Proc. Natl. Acad. Sci. U.S.A.)

point mutations in Unc13 functional domains (C1 domain and C2B domain) mEOS2.1 tagged Unc1

https://pubmed.ncbi.nlm.nih.gov/40833403