I am trying to silence a gene in nervous system, it is very difficult.
shRNA may be useful.
Is any protocol to make shRNA?
I would be grateful if someone helps.
Well, for hairpin RNAi transgene protocols, in addition to the description in the first paper, by Tavernarakis et al, there was a Worm Breeder’s article (page 18) from the Jorgenson lab, and a couple of recent papers in Gene.