SL1 splicing....

Model Organism: Worms Scientific Discussion
1

Hi Everyone,

Really basic question about SL1 transplicing:

When an exon is “SL1-spliced” does it always remove the upstream exon? I’m working with a large multi-exon gene that has many of these SL1 sites throughout. I’m wondering if the SL1 sites on internal exons result in the removal of upstream exons because they are effectively splice to a new leader. Or, do these exons get joined and just use the SL1 as an intermediate. I find the SL1 splicing thing very confusing.

Thanks!

2

I believe that the upstream exons are removed when the SL1 is spliced in.

You can read over the WormBook chapter on transplicing for clarification.

http://www.wormbook.org/chapters/www_transsplicingoperons/transsplicingoperons.html

Janet

3

Thanks so much. I read the chapter and it wasn’t explicitly stated, which is why I was confused… A few other papers helped, and I think you’re right. Its really the only way to make sense of it.