I am not quite sure exactly how it works except that it basically suffocates the worm. I can not use it as it has horrid problems. I lose significant GFP expression because the body totally breaks down.
I used NaAzide, but I realize now that it has such bad effects. I am actually wondering if there is something better to fix with besides paraformaldehyde. I think I am losing a lot of signal using paraformaldehyde. I read the blogs and checked my pH, and it was 7.4.
The GFP signal is bright when I don’t fix, but I can’t see exactly localization patterns without fixing.
I am working with worms at the 2 fold- 3 fold stage. Most members in my lab work on stages before that so I am the first that has to worry about fixing embryos.
Hello. There seem to be a fair number of labs that switch to full on antibody staining protocols using anti-GFP antibodies if they must fix their embryos.