specific promoter for V5.pa blast cells

Model Organism: Worms Scientific Discussion
1

Hi, all

I don’t know if someone knows specific promoter for V5.pa blast cells.
Thanks a lot!!

2

Hi, I can suggest the egl-17 promoter which is active in Q and V5, and gets upregulated in V5.pa and its descendants.

Best of luck!

J

3

Thanks a lot!
I also used egl-17, and it seems that it highly expressed in Q blast but not V5.pa descendants. I will try again to see.
In fact I want to monitor the blast cell of PVD which is in V5.pa lineage. any other suggestions?

Thanks!

4

You might take a look at lin-32, or ask those with experience using it. It’s required for V5.pa neuroblast fate, it’s likely cell-autonomous, and the expression information I could find in a very rapid look suggested it’s not expressed in the hypoderm - so it really ought to be functioning, and so expressed, in the V5.pa. Whether there’s a good reporter to reiterate this, I don’t know.

5

Thanks a lot. I know lin-32 is expressed in Q lineage. I am trying it. I also don’t know if it is a good marker. Thanks a lot anyway.