dear worms,
could anybody provide a protocol or hints for staining of the microtubule cytoskeleton in adults, especially in muscle cells?
i have tried antibodies against alpha tubulins by methanol/aceton fixation and by PFA fixation, without success. GFP fusion strains will not really help me, as i am already working in a transgenic GFP background.
search (google, pubmed and textpresso) was unsuccessful so far.
thanks,
mic