Hi, I have three sterile lines to map using the CloudMap pipeline. Currently I am a bit worried about the strategy to use for providing enough material out of sterile recombinants.
Does anyone have already encounter this issue, having any ideas of which strategies, kits would be the best? Best wishes.
We routinely sequence steriles from 50 handpicked animals, using a combined shearing/gDNA isolation protocol and ChIP-Seq library prep (or other low input kit). PM if you’d like a detailed protocol.
Harold