I’m currently going through the exciting task of ordering primers and figuring out which enzymes to use for some of my crosses involving point mutations. I already use ApE to help me with this, but it still requires time to find that perfect 1 cutter that produces a reasonable sized band and already exists in the lab enzyme stash.
This got me thinking…perhaps some sort of genotyping assistance can be displayed on the allele variation page? It’s pretty fantastic that most point mutations are reported on Wormbase, but I think it’d make life a bit easier if it told you which restriction enzyme would be best for genotyping that particular allele. Would this be practical? I feel like it could be implemented through programming tools or even user submitted data.
