Hi, I’m looking to degrade a protein of interest in the germline but can’t use the existing sun-1p::TIR1:mRuby MosCI strains that are on Chr’s II and IV because the genes I’m working on are on those chromosomes. Therefore I was wondering if anyone has made a variant of this strain in which sun-1p::TIR-1 is inserted into the MosCI site on Chr I that they wouldn’t mind sharing with me. Thanks so much in advance
It’s not a sun-1p strain, but we made mex-5p TIR-1 strains knocked into the ttTi4348 and ttTi5605 sites for our Ashley et al Genetics paper. All strain at CGC. Those could work well for you.
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