Supplemental plates of the Ahringer library

Model Organism: Worms Scientific Discussion
1

Hi all,
we are having massive problems with thawing and culturing bacterial clones from the supplemental plates of the Ahringer library.
The “core” initial library was no trouble at all.
I was wondering if anyone has had this experience as well. If this is a general problem, it would be good to know before we contact Source BioScience.

Best,
Martin

2

it is a general problem. we ordered them also and found the same thing. my understanding is that source bioscience was not helpful when contacted since they said those clones were confirmed dead.

3

were they dead or just pining for the fjords?

4

some claimed to be getting better, but were then clubbed into submission