Supplemental plates of the Ahringer library

Hi all,
we are having massive problems with thawing and culturing bacterial clones from the supplemental plates of the Ahringer library.
The “core” initial library was no trouble at all.
I was wondering if anyone has had this experience as well. If this is a general problem, it would be good to know before we contact Source BioScience.


it is a general problem. we ordered them also and found the same thing. my understanding is that source bioscience was not helpful when contacted since they said those clones were confirmed dead.

were they dead or just pining for the fjords?

some claimed to be getting better, but were then clubbed into submission