As a part of migration from the Worm Community Forum, this post was manually created on behalf of the user YeHong; when they join the forum here, they can claim this post by replying to this topic.
I have a question about doing yeast two hybridization for proteins from C. elegans.
I have checked several papers and all of them did the Y2H at 30 degree.
Is it better to do it at lower temperature such as 25 degree to allow correct folding of worm protein,
Since C. elegans normally grow at 20-25 degree, and we usually express worm proteins at low temperature such as 16 degree?