You can find the new papers here: FlyBase:Papers with technical advances - FlyBase Wiki
Protocol to analyze 3D neurodegenerative vacuoles in Drosophila melanogaster. (STAR Protoc)
new protocol for quantifying vacuoles in fly brain
Low-input PacBio sequencing generates high-quality individual fly genomes and characterizes mutational processes. (Nat. Commun.)
A new PacBio sequencing library construction method named LILAP.
Dissection and Immunolabeling of the Central and Peripheral Nervous System of Drosophila Larvae. (Cold Spring Harb. Protoc.)
Technical guidance on dissection and immunolabeling of 3rd instar larvae
Proximity Ligation Assay (PLA) for Fillets of Drosophila Larvae. (Cold Spring Harb. Protoc.)
Technical description and protocol for proximity ligation assay in 3rd instar larvae
Whole-Larva Cryosectioning and Immunolabeling of Drosophila Larvae. (Cold Spring Harb. Protoc.)
Technical description and protocol for cryo-sectioning of Drosophila larvae for greater imaging resolution
Con-DAM: Simultaneous measurement of food intake and sleep in Drosophila at the single fly resolution. (MicroPubl Biol)
Con-DAM integrates the Con-Ex (Consumption-Excretion) assay and the DAM (Drosophila Activity Monitor) into a single unit. We expect Con-DAM to serve as an easy method for various purposes that require quantifying food consumption and sleep/activity in the same individual flies.
Species identification of livefood flightless fly (Torinido-shoujoubae) through DNA barcoding. (Ecol. Evol.)
DNA barcoding is a reliable method for species identification, while traditional morphological identification struggles with cryptic species. DNA barcoding democratizes scientific research by enabling reproducibility and consistency across global studies.
A protocol for measuring plasma membrane tension in the Drosophila ovary using fluorescence lifetime imaging microscopy. (STAR Protoc)
This protocol provides details on studying plasma membrane stretching in developing Drosophila ovarian cysts using Flipper-TR dye and fluorescence lifetime imaging microscopy.
A nuclear architecture screen in Drosophila identifies Stonewall as a link between chromatin position at the nuclear periphery and germline stem cell fate. (Genes Dev.)
- Screen for nuclear architecture proteins that are required for peripheral chromatin positioning 2) Interactors of Stwl in cultured cells 3) RNA-seq from female GSC-like cells upon Stwl overexpression 4) CUT&RUN data for genomic targets of Stwl in female GSC-like cells.
LarvaTagger: manual and automatic tagging of Drosophila larval behaviour. (Bioinformatics)
Software for behavioral phenotyping
Chromatin-associated cohesin turns over extensively and forms new cohesive linkages in Drosophila oocytes during meiotic prophase. (Curr. Biol.)
Smc1 tag-switch transgene. mCherry tag flanked by FRT sites. Superfolder GFP follows the mCherry cassette. Before FRT expression, Smc1-mCherry is expressed. FRT excision results in expression of Smc1-mCherry. So one can “switch” the tag by inducing FRT expression.
Chromatin-associated cohesin turns over extensively and forms new cohesive linkages in Drosophila oocytes during meiotic prophase. (Curr. Biol.)
Smc1 tag-switch transgene. mCherry tag flanked by FRT sites. Superfolder GFP follows the mCherry cassette. Before FRT expression, Smc1-mCherry is expressed. FRT excision results in expression of Smc1-mCherry. So one can “switch” the tag by inducing FRT expression.
Females translate male mRNA transferred during mating. (iScience)
We developed a proteomic method (VESPA, Variant Enabled SILAC Proteomic Analysis) to test the hypothesis that Drosophila male seminal fluid RNA is translated by females.
PI4P-mediated solid-like Merlin condensates orchestrate Hippo pathway regulation. (Science)
transgenic flies with tub-Mer-mNG, tub-MermutCC-mNG, tub-MermutCC-DIX-mNG, tub-MermutCC-FUS-mNG, or tub-GFP-2xOsh2-PH (PI4P reporter)
Drosophila melanogaster sperm turn more oxidative in the female. (J. Exp. Biol.)
characterisation of sperm metabolism during sperm storage application of fluorescence lifetime imaging microscopy
Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability. (PNAS Nexus)
cMyc-wt and cMyc-5P were generated in this study.