The FlyBase Papers with Technical Advances page has updated with March submissions

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Systematic screening of enhancer-blocking insulators in Drosophila identifies their DNA sequence determinants. (Dev. Cell)

Tonelli et al. develop insulator-seq a new high-throughput functional screening assay for enhancer-blocking insulators in Drosophila cells. Insulator-seq provides insights into how insulators are encoded in DNA.

Capturing continuous, long timescale behavioral changes in Drosophila melanogaster postural data. (PLoS Comput. Biol.)

Long timescale, fine-grained behavioral monitoring

A collection of split-Gal4 drivers targeting conserved signaling ligands in Drosophila. (G3 (Bethesda))

A collection of knock-in Split-Gal4 lines for ligands for major signaling pathways

Nutrient status alters developmental fates via a switch in mitochondrial homeodynamics. (Nat. Commun.)

We collected RG samples for RNA sequencing from the three groups of larvae at 108 h AEL, pre-NRC starvation, and post-NRC starvation, with three biological replicates in each group.

Interchromosomal contacts between regulatory regions trigger stable transgenerational epigenetic inheritance in Drosophila. (Mol. Cell)

An approach called 3D-CIS to induce long-range and interchromosomal locus-specific 3D contacts is described. For 3D-CIS, Tet operators and Lac operators are inserted. A fusion protein combining the Lac and Tet repressors with GFP binds both loci and forces their interaction.

Astrocyte-to-neuron H2O2 signalling supports long-term memory formation in Drosophila and is impaired in an Alzheimer’s disease model. (Nat Metab)

Introduction and characterization in Drosophila of the ultrasensitive H2O2 sensor roGFP2-Tsa2DCr originally developed by the lab of Tobias Dick (Morgan et al. (2016) Nat. Chem. Biol. 12, 437–443). This sensor allows imaging physiological changes in H2O2 levels.