Here’s the new papers: FlyBase:Papers with technical advances - FlyBase Wiki
Valve cells are crucial for efficient cardiac performance in Drosophila. (PLoS Genet.)
valve cell imaging
Aminergic and peptidergic modulation of insulin-producing cells in Drosophila. (eLife)
Broad collection of aminergic and peptidergic inputs to Insulin-Producing Cells, including transcriptome, connectome, and functional physiology data.
Sensitive and Accurate Proteome Profiling of Embryogenesis Using Real-Time Search and TMTproC Quantification. (Mol. Cell. Proteomics)
New proteomics method applied to wt D. melanogaster embryonic time-series.
A new Drosophila melanogaster research resource: CRISPR-induced mutations for clonal analysis of fourth chromosome genes. (G3 (Bethesda))
New stocks with 4th chromosome mutations
Pan-PTM profiling identifies post-translational modifications associated with exceptional longevity and preservation of skeletal muscle function in Drosophila. (NPJ Aging)
Post-translational modifications associated with protection from age-related skeletal muscle dysfunction in Drosophila strains with extreme longevity compared to the parental control strain.
Cell-Type Specific Variation in X-Chromosome Dosage Compensation in Drosophila. (MicroPubl Biol)
Using the Fly Cell Atlas (388,918 nuclei), we analyzed cell-type-specific X-chromosome dosage compensation. The resource includes RoX1/RoX2 expression data to support future research on cell type–dependent dosage compensation.
Exploiting the SunTag system to study the developmental regulation of mRNA translation. (J. Cell Sci.)
New tools and strategies to exploit SunTag imaging in the Drosophila embryo to compare translation of maternal and zygotic transcripts, investigate ribosome pausing and study translation of secreted proteins at the ER.
Bioinformatics Analysis Identifies Sequence Determinants of Enzymatic Activity for the PHARC-Associated Lipase ABHD12. (Biochemistry)
CG15111 is identified as an ortholog of human ABHD12. Generation of a expression construct for the Serine Hydrolase CG15111 in S2 cells and confirming its expression and enzyme activity by ABPP. Expression of inactive variant CG15111(S241A) in S2 cells.
Pumilio differentially binds to mRNA 3’ UTR isoforms to regulate localization of synaptic proteins. (EMBO Rep.)
xRIP-seq analysis of Pum in Drosophila heads. Samples are input and Pum IP. Synaptosome purification in Drosophila heads followed by proteomics analysis and mRNA sequencing analysis. Samples are input (head lysate) and synaptosome fraction.
An improved FLARE system for recording and manipulating neuronal activity. (Cell Rep Methods)
This study introduces cytoFLARE, an improved FLARE system for time-gated labeling and manipulation of activated neurons in Drosophila. Using cytoFLARE, reactivation and inhibition of neurons activated during nociception were achieved.