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Gut-to-brain regulation of Drosophila aging through neuropeptide F, insulin, and juvenile hormone. (Proc. Natl. Acad. Sci. U.S.A.)
We developed a method to directly quantify secreted neuropeptides in adult hemolymph.
Tissue-specific knockout in the Drosophila neuromuscular system reveals ESCRT’s role in formation of synapse-derived extracellular vesicles. (PLoS Genet.)
Cas9s for motoneurons, muscle and glia.
Transgenic sensors reveal compartment-specific effects of aggregation-prone proteins on subcellular proteostasis during aging. (Cell Rep Methods)
Here, by targeting the misfolding-prone FlucDM luciferase to the cytoplasm, mitochondria, and nucleus, we established transgenic sensors to examine subcellular proteostasis in Drosophila. We have sent copies of these stocks to the Bloomington stock center.
Mifepristone and rapamycin have non-additive benefits for life span in mated female Drosophila. (Fly)
Non-invasive in vivo assay of mitophagy reporter
Pathogenic tau induces an adaptive elevation in mRNA translation rate at early stages of disease. (Aging Cell)
We provide a new dataset of the translatome in control and tau transgenic Drosophila.
Rab11 suppresses neuronal stress signaling by localizing dual leucine zipper kinase to axon terminals for protein turnover. (eLife)
Various UAS-Wnd constructs and transgenic fly lines were generated during the study - including the serial deletion of Wnd-ORF, the Wnd transgenes with and without the untranslated regions, the point mutant Wnd transgene of C130S (Cysteine 130 to Serine 130 replacement).
Matrix metalloproteinases: Master regulators of tissue morphogenesis. (Gene)
Newly developed transgenic lines used in this study help in Live imaging of Muscle stem cells in Drosophila together with neighbouring tissues.
A genome-engineered tool set for Drosophila TGF-β/BMP signaling studies. (Development)
This paper provides a collection of new, molecularly defined null alleles of the TGF-β/BMP components as well as a collection of endogenously epitope- or fluorescent-tagged pathway components. Furthermore, it reports the generation of a library of HA protein binder-based tools.
Semi-Automated Assessment of Long-Term Olfactory Habituation in Drosophila melanogaster Using the Olfactory Arena. (Bio Protoc.)
This protocol allows quantitative and detailed behavioral measurements obtained by video recording with little interference or manual skill from the experimenter, that allows sophisticated forms of offline analysis and that can be shared with the scientific community.
Target gene responses differ when transcription factor levels are acutely decreased by nuclear export versus degradation. (Development)
Dorsal tagged with LEXY, a blue light inducible export system (optogenetic tag)
Detecting Native Protein-Protein Interactions by APEX2 Proximity Labeling in Drosophila Tissues. (Bio Protoc.)
This protocol provides a detailed workflow for mapping the endogenous interactome networks of Drosophila ovarian proteins. It employs CRISPR/Cas9 knock-in to tag endogenous genes with ascorbate peroxidase 2 (APEX2) and utilizes optimized proximity labeling technology.
Scale invariance in early embryonic development. (Proc. Natl. Acad. Sci. U.S.A.)
This paper reports a new approach based on information theory to test whether spatial expression patterns of one gene, or a combination of genes, scale with the embryo length. The method is independent of the functional form of the spatial gene expression profiles.