The new papers can be found here: https://wiki.flybase.org/wiki/FlyBase:Papers_with_technical_advances#Oct_2024
Protocol for Imaging the Same Class IV Neurons at Different Stages of Development. (Bio Protoc.)
This protocol involves imaging the same larvae class IV neurons without the use of anesthetics and for a short duration of time using spinning disk confocal microscope.
Selective optogenetic inhibition of Gαq or Gαi signaling by minimal RGS domains disrupts circuit functionality and circuit formation. (Proc. Natl. Acad. Sci. U.S.A.)
LexAop-PiGM-Iq: optogenetic tool to disrupt G protein alpha subunit q signaling through membrane recruitment of a minimal regulator of G protein signaling (RGS) domain
Glial expression of Drosophila UBE3A causes spontaneous seizures that can be modulated by 5-HT signaling. (Neurobiol. Disease)
We present a Hidden Markov Model trained to detect seizure-related behavioral phenotypes in a model for Dup15q syndrome.
A sexually transmitted sugar orchestrates reproductive responses to nutritional stress. (Nat. Commun.)
Synthetic venerose. 1-O-[4-O-(2-aminoethylphosphate)-β-D-galactopyranosyl]-x-glycerol in a R and S isomer mix was custom synthesized by WuXi AppTec Co (China).
Optimization of hybridization chain reaction for imaging single RNA molecules in Drosophila larvae. (Fly)
Optimisation of hybridisation-chain reaction (HCR) for imaging single molecules of RNA in drosophila larvae. Includes a free-to-use, customisable probe designer tool and protocol details.
Redesigning the Drosophila histone gene cluster: an improved genetic platform for spatiotemporal manipulation of histone function. (Genetics)
New platform for generating histone mutant genotypes for use in epigenetic studies.
Spatial organization of translation and translational repression in two phases of germ granules. (Nat. Commun.)
To reach the resolution required to resolve germ granule organization by super resolution microscopy, the distance between the sample and the objective was reduced by slicing the posterior of embryos.
https://pubmed.ncbi.nlm.nih.gov/39271704
Dachsous and Fat coordinately repress the Dachs-Dlish-Approximated complex to control growth. (J. Cell Biol.)
This paper includes a new type of reagents to study protein complex and protein recruitment in cell culture system.
Transcriptomic analysis of meiotic genes during the mitosis-to-meiosis transition in Drosophila females. (Genetics)
Isolation of mitotic and meiotic cell populations by FACS based on the restrictive expression pattern and properties of Bam::GFP and Wcd::RFP transgenic proteins. This allowed processing the RNA for high-throughput sequencing.
Interactions of drosophila cryptochrome. (Photochem. Photobiol.)
This paper shows that BoID technique can be used to identify light-dependent or -induced interaction using Drosophila cell line S2.
The conserved genetic program of male germ cells uncovers ancient regulators of human spermatogenesis. (eLife)
An interactive online application (Meiotic Navigator) compiling new functional data on male fertility and associated testicular microscopy images of 920 evolutionarily conserved spermatocyte genes is available at https://pages.igc.pt/meionav.