Hi there,
Does anyone out there know of any thermal tolerant species/mutants (c.elgans or similar) that might be quite comfortable around or above 37C? I have been actually pulling my hair out trying to find some that are ok at this temperature. If anyone has ANY information please let me know so that I can stop this vicious habit.
phact_searcher
According to the following paper you could maybe use this Panagrellus species.
1: FEMS Microbiol Lett. 2005 Sep 1;250(1):77-83.
The nematode Panagrellus redivivus is susceptible to killing by human pathogens
at 37 degrees C.
Laws TR, Smith SA, Smith MP, Harding SV, Atkins TP, Titball RW.
Defence Science and Technology Laboratory, Biomedical Sciences, Porton Down,
Salisbury, Wiltshire SP4 OJQ, UK. trlaws@dstl.gov.uk
Caenorhabditis elegans has been used as a host for the study of bacteria that
cause disease in mammals. However, a significant limitation of the model is that
C. elegans is not viable at 37 degrees C. We report that the gonochoristic
nematode Panagrellus redivivus survives at 37 degrees C and maintains its life
cycle at temperatures up to and including 31.5 degrees C. The C. elegans
pathogens Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, but
not Yersinia pseudotuberculosis, reduced P. redivivus lifespan. Of four strains
of Burkholderia multivorans tested, one reduced P. redivivus lifespan at both
temperatures, one was avirulent at both temperatures and two strains reduced P.
redivivus lifespan only at 37 degrees C. The mechanism by which one of these
strains killed P. redivivus at 37 degrees C, but not at 25 degrees C, was
investigated further. Killing required viable bacteria, did not involve bacterial
invasion of tissues, is unlikely to be due to a diffusible, bacterial toxin and
was not associated with increased numbers of live bacteria within the intestine
of the worm. We believe B. multivorans may kill P. redivivus by a
temperature-regulated mechanism similar to B. pseudomallei killing of C. elegans.
PMID: 16040202 [PubMed - indexed for MEDLINE]