Hello,
I plan to do a thrashing assay on my wild type and mutant worms which are grown from the egg stage in plates containing .2mm paraquat.
In thrashing assay, I plan to put the worm on each well containining 50ul M9 and then manually count the thrashes.
My question, will I add .2mm paraquat in this well-M9 also? Or it will be only M9 without any dose of paraquat?
Any suggestions will be highly appreciated…