Hello there! I am new to the C. elegans research. I have been going through methods to create transgenic strains and RNAi techniques. The most common ways to create transgenic strains are through microinjection or biolistic bombardment. And RNAi through feeding. My question may sound a bit idiotic but I want to know if there is any way an expression vector could be delivered to C. elegans throung feeding transformed bacteria. Thank you for your help.
I have never heard of this happening, and the C. elegans gut has a lot of NUC-1 DNase activity.
Quite a long time ago I saw a report saying they’d transformed C. elegans by electroporation, but as I recall it was described as inefficient and so far as I know has never been published (there is a recent paper on delivering dsRNA and CRISPR guide RNA by electroporation). If I recall, the report of transforming C. elegans by electroporation was a California State Science Fair presentation, maybe 15 years ago.
Thank you for your response.