Hi all, I was planning on doing some immunofluorescence experiments on a lowly expressed transcription factor. (I usually need to use the super sensitive femto HRP substrates to see the protein on western blots). I’ve inserted a 3xFLAG epitope into the endogenous locus with co-conversion, and was planning to do anti-FLAG staining. I was wondering if anyone had tried tyramide signal amplification in C.elegans when fixing whole worms with the Finney-Ruvkun method? I was wondering if fixation process permeabilized the cuticle enough to get the antibody-HRP conjugate into the animal?