For those using MosSCI as genomic editing and single copy insertion, I was wondering if you have seen any particular phenotypes with the unc-119 background? Have you decided to change to Minimos to avoid having unc-119 in the background?
Hello Vicky,
you should look at our micropub Omi, Shizue et al. (2021) unc-119 mutants have an increased fungal spore adhesion that is not rescued by Cb-unc-119 WBPaper00060810. We do now all our single insertion through Cas9
Good luck,
Nathalie