Does any know the sequence variation of unc-29(e1072)? It would be great if you can share that information with me.
Thank you!
I assume you’ve seen Kim et al 2001?
By sequencing the complete coding regions of the unc-29 mutant alleles, we determined that the unc-29(x29) mutation introduced a nonsense mutation (tat → taa) within the fourth transmembrane α-helix. unc-29(e193) was found to be a missense mutation (cca → tca) that changes a universally conserved proline residue at position 258 to a serine; the sequence alteration in the unc-29(e1072) allele could not be identified.Obviously there’s a chance they slipped up somewhere, but this suggests it’s noncoding, and so could be hard to find and to prove.