Using PA14 (P. aeruginosa) on NGM plates

Model Organism: Worms Methods & Reagents
1

Hello All,

I recently attempted to use P. aeruginosa (PA14) for some immunity experiments, but I ran into a weird problem. When I seeded my NGM plates with the PA14, the agar turned green. Is this normal or did my PA14 get mutated? Also, if I need to get a hold of a new batch of PA14, does anyone know where I can purchase this from? Any information you might share with me would be most appreciated.

Sincerely,

S. Vetrone

2

Don’t worry, its normal. If you doing immunity assays you may want to consider doing them on peptone-rich plates rather than straight. Although it doesn’t seem necessary, it is fairly typical in the community. Also, there seems to be a distinction in pathogenesis between assays done on NGM vs Peptone-rich NGM.