If you want to UV kill OP50 seeded on NGM plates should you leave the OP50 dry overnight and form a lawn before exposing to UV or should you concentrate the OP50 (10x for example), seed and immediately UV kill, will the worms have enough food to develop normally?
Also in the same way I’m wondering about inclusion of ingredient, as whether you mix it with the OP50 culture or include it in the NGM agar the overnight incubation will allow time for the OP50 to interact with the ingredient in question before you UV kill the next day.
Hope this makes sense and you can share some wisdom!