We want to overexpress and isolate a C. elegans protein that has very low expression levels. What vector or promoter would you recommend me?
Thanks!
Hi,
It depends in which cell type you want to express the protein of interest.
If you don’t care about the cell type, you could express the gene ubiquitously, using a ribosomal promoter like rps-27 or rpl-28 for example.
Thaks for the advice!
The heat shock promoters are another option, and also provide the flexibility of inducible expression.
-Harold