Hi
I am quite new to this forum so please forgive me if I violate any laid down rule (I’m assuming there are quite a few)
I intend to do a western blot (WB) on L1 worms in order to quantify SNA-2, SNA-1 and SUT-1 proteins in a strain that I made. The problem is that I do not know how many worms I will have to pick for this assay so as to get a good signal.
Has anyone done a WB on L1 worms before? How many worms will you suggest I pick, to get a good signal (assuming the primary antibody I want to use is already optimised)?
I will appreciate any information that will make my assay go smoothly, as this is the first time I will be doing this assay.